diff --git a/mite_data/data/65e9db92-81f5-11f1-a062-b15f8c759613.json b/mite_data/data/65e9db92-81f5-11f1-a062-b15f8c759613.json new file mode 100644 index 00000000..e4c30b60 --- /dev/null +++ b/mite_data/data/65e9db92-81f5-11f1-a062-b15f8c759613.json @@ -0,0 +1,67 @@ +{ + "accession": "MITE9999999", + "status": "pending", + "comment": "DnrK also known as Carminomycin 4-O-methyltransferase is involved in the biosynthesis of anthracycline daunorubicin.", + "changelog": [ + { + "version": "1", + "date": "2026-07-17", + "contributors": [ + "0009-0008-8086-5325" + ], + "reviewers": [ + "BBBBBBBBBBBBBBBBBBBBBBBB" + ], + "comment": "New entry." + } + ], + "enzyme": { + "name": "DnrK", + "description": "O-methyl transferase", + "references": [ + "doi:10.1128/jb.175.12.3900-3904.1993" + ], + "databaseIds": { + "uniprot": "Q06528", + "genpept": "AAA26712.1" + }, + "cofactors": { + "organic": [ + "SAM" + ] + } + }, + "reactions": [ + { + "tailoring": [ + "Methylation" + ], + "description": "DnrK catalyzes the methylation of the 4-hydroxyl group in tetracyclic ring system of carminomycin to form daunorubicin", + "reactionSMARTS": "[#6:1]/[#6@@:2]1-[#8:7]-[#6@@:6](\\[#8:8]/[#6@@:9]2-[#6:18]3:[#6:13](:[#6:14](-[#8:31]):[#6:15]4-[#6:24](=[#8:25])-[#6:23]5:[#6:26]:[#6:27]:[#6:28]:[#6:29](-[#8:30]):[#6:22]:5-[#6:20](=[#8:21])-[#6:16]:4:[#6:17]:3-[#8:19])-[#6:12]-[#6@@:11](/[#8:35])(\\[#6:32](-[#6:34])=[#8:33])-[#6:10]-2)-[#6:5]-[#6@:4](/[#7:36])-[#6@@:3]-1/[#8:37]>>[#6:1]/[#6@@:2]1-[#8:7]-[#6@@:6](\\[#8:8]/[#6@@:9]2-[#6:18]3:[#6:13](:[#6:14](-[#8:31]):[#6:15]4-[#6:24](=[#8:25])-[#6:23]5:[#6:26]:[#6:27]:[#6:28]:[#6:29](-[#8:30]-[#6]):[#6:22]:5-[#6:20](=[#8:21])-[#6:16]:4:[#6:17]:3-[#8:19])-[#6:12]-[#6@@:11](/[#8:35])(\\[#6:32](-[#6:34])=[#8:33])-[#6:10]-2)-[#6:5]-[#6@:4](/[#7:36])-[#6@@:3]-1/[#8:37]", + "reactions": [ + { + "substrate": "CC(=O)[C@]1(O)Cc2c(O)c3c(c(O)c2[C@@H](O[C@H]2C[C@H](N)[C@H](O)[C@H](C)O2)C1)C(=O)c1c(O)cccc1C3=O", + "products": [ + "COc1cccc2c1C(=O)c1c(O)c3c(c(O)c1C2=O)C[C@@](O)(C(C)=O)C[C@@H]3O[C@H]1C[C@H](N)[C@H](O)[C@H](C)O1" + ], + "isIntermediate": false, + "description": "DnrK has a broad substrate specificity and can methylate other anthracyclines in addition to carminomycin, for instance \u03f5-rhodomycin T" + } + ], + "evidence": { + "evidenceCode": [ + "Heterologous expression", + "In vitro assay" + ], + "references": [ + "doi:10.1128/jb.175.12.3900-3904.1993", + "doi:10.1074/jbc.M407081200" + ] + }, + "databaseIds": { + "rhea": "38311", + "ec": "2.1.1.292" + } + } + ] +} \ No newline at end of file