vflank is the variant-aware, optionally patient-specific, masked-flank front-end of a ddPCR assay-design pipeline. It extracts the sequence flanking each variant and masks positions that would compromise a primer/probe, then emits clean targets in designer-native formats.
A single-page web front-end for vflank — paste, upload, or type a handful of variants and get back masked ddPCR target sequences (and optional Primer3 input), with no install and no patient data leaving the browser.
This R script retrieves upstream and downstream DNA flanking sequences for given rsIDs or a list of rsIDs. It utilizes online databases to download sequence data and miscellaneous data about the SNP.
A Tool for Flanking Sequence Interpretation and Visualization in Rice, Maize, and Soybean
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